Fig S1. Evaluation of the statistical properties of scaffolds of Ca. M. hydrogenus Bin16 (a), Bin11 (b) and Bin74 (c).

Histograms in the left show the GC-content, tetranucleotide distances (TD) and coverage error of scaffolds within Bin16, Bin11 and Bin74. The length (y-axis) of each scaffold was plotted against deviation from the mean GC content, tetranucleotide signature, or sequencing coverage (x-axis) in the scatterplot. The dashed red lines show the 95th percentile calculated by RefineM v0.0.23 (4). Scaffolds in red contain methanogenesis-associated genes.