Figure 3.tif (9.71 MB)
Fig 3 QRFP-producing neurons are distributed in and around the LHA and innervated by fibers sent by arcuate neurons.
figure
posted on 2016-10-20, 01:17 authored by Kitaro OkamotoKitaro Okamoto, Miwako Yamasaki, Keizo Takao, Shingo Soya, Monica Iwasaki, Koh Sasaki, Kenta Magoori, Iori Sakakibara, Tsuyoshi MiyakawaTsuyoshi Miyakawa, Michihiro Mieda, Masahiko Watanabe, Juro Sakai, Masashi Yanagisawa, Takeshi SakuraiTakeshi SakuraiA, Triple immunofluorescent image of QRFP neurons (GFP, green), orexin-ir neurons (blue) and MCH-ir neurons (red) in
hypothalamic slice of QRFP+/- mouse. A1, QRFP neurons (GFP, green) of perifornical region of QRFP+/- mouse. Fx, fornix. A2, Triple immunofluorescent image of QRFP neurons (GFP, green), orexin-ir neurons (blue) and MCH-ir neurons (red) in same section as in A1. A3, High power view of yellow rectangular region in A2.
hypothalamic slice of QRFP+/- mouse. A1, QRFP neurons (GFP, green) of perifornical region of QRFP+/- mouse. Fx, fornix. A2, Triple immunofluorescent image of QRFP neurons (GFP, green), orexin-ir neurons (blue) and MCH-ir neurons (red) in same section as in A1. A3, High power view of yellow rectangular region in A2.
B, QRFP neurons receive innervations from AgRP-immunoreactive (ir) fibers. Double
immunofluorescence for QRFP neurons (GFP, green) and AgRP-ir fibers (red) showing dense innervation by AgRP neurons in the hypothalamic area. B1-2, Low power (B1) and
high power (B2) views of double-labeling immunofluorescent image of GFP (green) and AgRP immunoreactivity in hypothalamic area. B3, double-labeled pre-embedding
immunoelectron microscopic image of AgRP (diffuse precipitates) and GFP (metal particles) showing a symmetrical synapse between a QRFP neuron and an AgRP
neuron. B4, High power view of immunoelectron microscopic image showing a synapse between a dendrite of a QRFP neuron and a terminal of an AgRP fiber. White arrowheads indicate the edge of postsynaptic density of symmetrical synapse.
immunofluorescence for QRFP neurons (GFP, green) and AgRP-ir fibers (red) showing dense innervation by AgRP neurons in the hypothalamic area. B1-2, Low power (B1) and
high power (B2) views of double-labeling immunofluorescent image of GFP (green) and AgRP immunoreactivity in hypothalamic area. B3, double-labeled pre-embedding
immunoelectron microscopic image of AgRP (diffuse precipitates) and GFP (metal particles) showing a symmetrical synapse between a QRFP neuron and an AgRP
neuron. B4, High power view of immunoelectron microscopic image showing a synapse between a dendrite of a QRFP neuron and a terminal of an AgRP fiber. White arrowheads indicate the edge of postsynaptic density of symmetrical synapse.
C, Innervation from NPY neurons to QRFP neurons. C1, Double immunofluorescent images of QRFP neurons (GFP, green) and NPY-ir fibers (red) in a hypothalamic slice of QRFP-/+ mouse. C2, High power view of region shown in yellow rectangle. C3-4, Low power (C3) and high power (C4) views of postembedding immunoelectron microscopy for NPY (immunogold) showing a symmetrical synapse between an NPY-ir terminal and a GFP-positive neuron. White arrowheads indicate the edge of postsynaptic density.
D, Close interaction between QRFP neurons and MSH-ir fibers. Presence of QRFP neurons (GFP, green) and MSH-ir fibers (red) in a hypothalamic slice of QRFP-/+ mouse, showing QRFP neurons are closely apposed to MSH-ir fibers. D1, Low power view of a hypothalamic slice stained with anti-MSH antibody. D2, High power view of
hypothalamic slice stained with anti-MSH (red) and anti-GFP antibodies. D3-4, Low power (D3) and high power (D3) views of double-labeling pre-embedding immunoelectron microscopic image showing MSH (diffuse precipitates) and GFP (metal particles). Black and white arrowheads indicate the edge of postsynaptic density of asymmetrical and symmetrical synapse, respectively.
D, Close interaction between QRFP neurons and MSH-ir fibers. Presence of QRFP neurons (GFP, green) and MSH-ir fibers (red) in a hypothalamic slice of QRFP-/+ mouse, showing QRFP neurons are closely apposed to MSH-ir fibers. D1, Low power view of a hypothalamic slice stained with anti-MSH antibody. D2, High power view of
hypothalamic slice stained with anti-MSH (red) and anti-GFP antibodies. D3-4, Low power (D3) and high power (D3) views of double-labeling pre-embedding immunoelectron microscopic image showing MSH (diffuse precipitates) and GFP (metal particles). Black and white arrowheads indicate the edge of postsynaptic density of asymmetrical and symmetrical synapse, respectively.
E, Double labeling immunofluorescent image showing expression of MC4 melanocortin receptor (MC4R,
red) in QRFP neurons (GFP, green) in a coronal section of an Qrfp-/+ slice.
red) in QRFP neurons (GFP, green) in a coronal section of an Qrfp-/+ slice.
