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Faujasites Incorporated Tissue Engineering Scaffolds for Wound Healing: In Vitro and In Vivo Analysis
journal contribution
posted on 2013-11-13, 00:00 authored by Neethu Ninan, Muthunarayanan Muthiah, In-Kyu Park, Anne Elain, Tin Wui Wong, Sabu Thomas, Yves GrohensExploring
the possibility of using inorganic faujasites in tissue
engineering scaffolds is a prospective approach in regenerative medicine.
Novel gelatin/hyaluronic acid (HA)/faujasite porous scaffolds with
low surface energy were fabricated by lyophilization. The pore size
of gelatin/HA scaffold was 50–2000 μm, whereas it was
greatly reduced to 10–250 μm after incorporation of 2.4%
(w/w) of faujasites in polymer matrix, GH(2.4%). Micro computed tomography
analysis showed that the porosity of GH(2.4%) was 90.6%. The summative
effect was ideal for growth of dermal fibroblasts and cellular attachment.
XRD analysis revealed that the embedded faujasites maintained their
crystallinity in the polymer matrix even though they interacted with
the polymers as indicated by FT-IR analysis. Coupling with effective
reinforcement of faujasites, GH(2.4%) demonstrated compression modulus
of 929 ± 7 Pa and glass transition temperature of 31 ± 0.05
°C. It exhibited controlled swelling and degradation, allowing
sufficient space for tissue regrowth. The latter is further supported
by capability of faujasites to provide efficient oxygen supply to
fibroblast cells. GH(2.4%) showed a cell viability of 91 ± 8%
on NIH 3T3 fibroblast cell lines. The in vivo studies on Sprague–Dawley
rats revealed its ability to enhance wound healing by accelerating
re-epithelization and collagen
deposition. These findings indicated its potential as excellent wound
dressing material.