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Facile Assembly/Disassembly of DNA Nanostructures Anchored on Cell-Mimicking Giant Vesicles
journal contribution
posted on 2017-08-25, 00:00 authored by Ruizi Peng, Huijing Wang, Yifan Lyu, Liujun Xu, Hui Liu, Hailan Kuai, Qiaoling Liu, Weihong TanDNA
nanostructures assembled on living cell membranes have become
powerful research tools. Synthetic lipid membranes have been used
as a membrane model to study the dynamic behavior of DNA nanostructures
on fluid soft lipid bilayers, but without the inherent complexity
of natural membranes. Herein, we report the assembly and disassembly
of DNA nanoprisms on cell-mimicking micrometer-scale giant membrane
vesicles derived from living mammalian cells. Three-dimensional DNA
nanoprisms with a DNA arm and a cholesterol anchor were efficiently
localized on the membrane surface. The assembly and disassembly of
DNA nanoprisms were dynamically manipulated by DNA strand hybridization
and toehold-mediated strand displacement. Furthermore, the heterogeneity
of reversible assembly/disassembly of DNA nanoprisms was monitored
by Förster resonance energy transfer. This study suggests the
feasibility of DNA-mediated functional biomolecular assembly on cell
membranes for biomimetics studies and delivery systems.