FGF23 treatment led to changes in renal NaPi-IIa and Vdr protein expression.
Representative western blots of total renal protein homogenates from mice injected with either vehicle (Ctrl) or recombinant human FGF23 (rhFGF23) once a day for two consecutive days using antibodies against NaPi-IIa (A), full length Klotho (B), Cyp24a1 (C) and Vdr (D). The proteins of interest are shown in the top panels, whereas the corresponding β-actin signal is shown in the bottom image. Densitometric quantification was normalized to the corresponding β-actin values. Bar graphs represent mean ± SD (n = 5-6/group) of the normalized values and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05.