Extraction of human DNA replication timing patterns from discrete microarray data

Effective reproduction is essential for the survival and proliferation of any organism, from the birth of new offspring to the repro- duction of individual cells. Each portion of a cell’s DNA must be copied exactly once during the replication phase of its cell cycle to ensure viability. In humans, this is achieved by a complex pattern of replication origins and terminations along the chromosomes until the final product is realized. DNA Tiling Microarrays are utilized to assay discrete pools of DNA replicated during different parts of the replication phase. We present a generalized framework for analyzing this discrete timing data to recover a relatively continuous profile of the DNA replication timing. This approach can be used to assay DNA replication timing over a variety of human cell lines or extended to other organisms. PRIB 2008 proceedings found at: http://dx.doi.org/10.1007/978-3-540-88436-1 Contributors: Monash University. Faculty of Information Technology. Gippsland School of Information Technology ; Chetty, Madhu ; Ahmad, Shandar ; Ngom, Alioune ; Teng, Shyh Wei ; Third IAPR International Conference on Pattern Recognition in Bioinformatics (PRIB) (3rd : 2008 : Melbourne, Australia) ; Coverage: Rights: Copyright by Third IAPR International Conference on Pattern Recognition in Bioinformatics. All rights reserved.