Expression of effector molecules by Gag-specific CD4+ T cells from HIV elite controller LNs and blood.

<p><b>(A)</b> Flow cytometry plots (HIV elite controllers) illustrating the distribution of LN (red) and PB (blue) IFNγ+ Gag-specific CD4+ T cell responses between the T-bet<sup>dim</sup>Eomes- and T-bet<sup>hi</sup>Eomes+ compartment. Corresponding scatterplots showing the frequencies of T-bet<sup>dim</sup>Eomes- (left) and T-bet<sup>hi</sup>Eomes+ (right) cells of Gag-specific CD4+ T cells between LN and PB for HIV elite controllers. Flow plots (HIV elite controllers) of IFNγ+ Gag-specific CD4+ T cell response from LN and PB in relation to <b>(B)</b> perforin and <b>(C)</b> MIP-1α expression. Graphs represent the frequency of <b>(B)</b> perforin+ and <b>(C)</b> MIP-1α+ Gag-specific CD4+ T cells between LN and PB. <b>(D)</b> Flow plots and graphs demonstrating the expression pattern of perforin+Granzyme B+ memory CD4+ T cells in acute/early seroconverters (left graph). Right graphs show the frequency perforin+Granzyme B+ or T-bet expression out of total memory Ki-67+ CD4+ T cells. Median and IQR are shown for all scatter plots. Mann-Whitney tests were performed to compare differences between groups; *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001. All data-points are derived from the North-American cohort.</p>