Expression of effector molecules by Gag-specific CD4+ T cells from HIV elite controller LNs and blood.

(A) Flow cytometry plots (HIV elite controllers) illustrating the distribution of LN (red) and PB (blue) IFNγ+ Gag-specific CD4+ T cell responses between the T-bet^dimEomes- and T-bet^hiEomes+ compartment. Corresponding scatterplots showing the frequencies of T-bet^dimEomes- (left) and T-bet^hiEomes+ (right) cells of Gag-specific CD4+ T cells between LN and PB for HIV elite controllers. Flow plots (HIV elite controllers) of IFNγ+ Gag-specific CD4+ T cell response from LN and PB in relation to (B) perforin and (C) MIP-1α expression. Graphs represent the frequency of (B) perforin+ and (C) MIP-1α+ Gag-specific CD4+ T cells between LN and PB. (D) Flow plots and graphs demonstrating the expression pattern of perforin+Granzyme B+ memory CD4+ T cells in acute/early seroconverters (left graph). Right graphs show the frequency perforin+Granzyme B+ or T-bet expression out of total memory Ki-67+ CD4+ T cells. Median and IQR are shown for all scatter plots. Mann-Whitney tests were performed to compare differences between groups; *P < 0.05, **P < 0.01 and ***P < 0.001. All data-points are derived from the North-American cohort.