figshare
Browse
tbbb_a_1301804_sm1365.xls (55.5 kB)

Expression of a MORN repeat protein from Euplotes octocarinatus requires a +1 programmed ribosomal frameshifting

Download (55.5 kB)
dataset
posted on 2017-03-20, 09:27 authored by Lili Wei, Xuemei Zhao, Ruanlin Wang, Yuejun Fu, Baofeng Chai, Aihua Liang

Analysis of transcriptome revealed that a membrane occupation and recognition nexus (MORN) repeat protein-encoding gene of Euplotes octocarinatus (Eo-morn-9-31) was a candidate for programmed +1 ribosomal frameshifting (+1 PRF). In this study, a dual-luciferase assay was performed to detect its expression. The result showed that the MORN repeat protein (Eo-MORN-9-31) could be produced by the +1 PRF event during the process of translation in yeast and the frameshifting efficiency was about 4–5%. We further confirmed its reality by western blot and mass spectrometry. This study provided experimental evidence indicating that the expression of the Eo-MORN-9-31 of E. octocarinatus required the +1 PRF.

The Eo-morn-9-31 could produce a MORN repeat protein containing nine MORN repeats with an apparent molecular weight of 41.2 kD, or only encode a truncated protein (34.8 kD) depending +1 PRF.

History