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Expression analysis of stress responsive genes in AdLEA transgenic plants.

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posted on 2016-03-03, 08:09 authored by Akanksha Sharma, Dilip Kumar, Sumit Kumar, Sakshi Rampuria, Attipalli R. Reddy, Pulugurtha Bharadwaja Kirti

Relative transcript levels of few abiotic stress responsive genes (NtP5CS, NtAPX, NtERD10c, NtNCED, NtCAT, and NtSOD) in AdLEA transgenic line #2 compared with WT were analyzed by qRT-PCR in (A) untreated and (B) drought stress conditions. All the experiments were performed in triplicates and data represented as mean ± SD (n = 3; biological replicates). RNA from single leaf from each plant constitute one biological sample. Statistical analysis was performed with two-way ANOVA (*P<0.05, **P<0.001). The primer sequences used in this study are given in Table B in S1 File.

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