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Example on the slide 14262.

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posted on 2018-01-19, 18:59 authored by Fidéline Bonnet-Serrano, Marc Diedisheim, Roberto Mallone, Etienne Larger

Selected Fast Red-stained pixels and subsequent results. Given that these 3 different areas were evaluated on pictures of different sizes (full-resolution picture for Fast Red-stained area versus resized pictures for total and exocrine+endocrine pancreatic tissue areas), all the results were normalized by the whole slide surface to allow further calculation of the parameters of interest, i.e. ratio of non-exocrine-non-endocrine pancreatic tissue (total pancreatic tissue minus exocrine+endocrine pancreatic tissue) to total pancreatic tissue area and ratio of either α-or β-cell area to total pancreatic tissue area, further converted into mass by multiplication by pancreas weight when this data was available. To take into account the difference in tissue area between slides, the average calculated for each patient, on respectively 6 slides for the ratio of non-exocrine-non-endocrine pancreatic to total pancreatic tissue, 4 slides for the ratio of α- cell area to total pancreatic tissue ratio and 2 slides for the ratio of β-cell area to total pancreatic tissue ratio, was weighted according to the total pancreatic tissue area of each slide.

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