posted on 2018-02-09, 12:56authored byAngel MojarroAngel Mojarro, Julie Hachey, Gary Ruvkun, Maria T. Zuber, Christoper E. Carr
fastQ files with preserved headers containing channel information. The multiple files must first be combined into a single FastQ file prior to analysis (e.g., cat *.fastq > all_reads.fastq). Concatenated md5: 26ce52582bfcf24c41082ef44224107c
0.2 ng of B. subtilis DNA prepared with 1000 ng of Lambda DNA using the Oxford Nanopore Technologies ligation sequencing kit - LSK-SQK108.The library was then sequenced on a MinION Mark-1B sequencer and R9.4 flowcell for 48 hours and basecalled using ONTs Albacore v1.10 offline basecaller.