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Evaluation of genetic diversity in germplasm of paprika (Capsicum spp.) using random amplified polymorphic DNA (RAPD) markers
journal contribution
posted on 2017-10-21, 07:37 authored by Renganathan P, Ruíz-Alvarado C, Hernández-Montiel LG, Prasath Duraisamy, Rueda-Puente EOCapsicum spp. is one of the most important economical
horticulture crops due to its high consumption either by fresh vegetable
or dried spice. Molecular genetic markers offer a number of
applications in the genetic improvement of crop plants, which plays an
important role in the areas of plant classification and breeding
programs. The polygenetic characters of rare species, which are
difficult to analyze by traditional methods can, be analyzed easily and
classified by using molecular markers. In our study, genetic
relationships of twenty-two paprika species were examined to estimate
their genetic variations/similarities and to detect the polymorphism
present within and among the paprika species by using RAPD-PCR markers.
The results revealed that the maximum similarities among the 16 ICBD
lines were 100%. The ICBD 03 had 76% similarity compared with other ICBD
lines. The CC01 had comparatively low similarity with ICBD forms (30%),
followed by EC01 (28%), EC02 (33%), CC02 (35%), and Kt.Pl-19 (60%). The
similarity between EC01 and EC02 were 54%. Kt.Pl-19 showed different
similarities compared to CC01 (41%), CC02, EC01 (38%), EC02 (29%) and
ICBD 03 (40%). The different combinations were tried to optimize the
RAPD-PCR profile, which helped to assessing the
polymorphism/similarities within and among the Paprika germoplasms were
studied.
