Estimation of the N and P reserve abundance in the studied cells.

(A) and (B) Estimation of the reserves in the Nostoc sp. PCC 7118 cells. (C) and (D) estimation of the reserves in the Chlorella vulgaris IPPAS C-1 cells. (E) and (F) estimation of the reserves in the Desmodesmus sp. IPPAS S-2014 cells. (A), (C) and (E) estimation of the N reserves in the cells of P-starved cultures. (B), (D) and (F) estimation of the P reserves in P-sufficient cells sampled from the stationary phase cultures. The results expressed in relative area of the P-rich or N-rich inclusions are shown for each cell section (n = 18). Relative area values on the histograms are sorted in descending order (A), (C), (E) or in descending order within the following groups: in (B), both P reserves associated with PHB and P reserves not associated with PHB; only P reserves not associated with PHB; in (D) and (F), main P reserves in the vacuoles, no P reserves in the cytosol; P reserves both in the vacuoles and in the cytosol and the main P reserves are located in the vacuoles; P reserves both in the vacuoles and in the cytosol and the main P reserves are located in the cytosol; the main P reserves in the cytosol, no P reserves in the vacuoles. For the P-rich inclusions (B), (D), (F) subcellular localization is also shown.