Estimation of .

A: Scheme showing the reactions as used in the experimental setup of Pierce et al. [10]. States and reactions have the same meaning as in Fig 1B. Note that Cul1 and Fbxw7 are bound to fluorescent dyes.B: Fit of the model simulations to a single exponential function. Data points for the first 5 seconds were discarded to obtain a better fit. Kinetic parameters were taken from Table 1.