Fig 1.tif (501.05 kB)
Establishment of stable cells overexpressing IDH1 wild type and IDH1 R132H mutant.
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posted on 2017-01-04, 18:36 authored by Huixia Zhu, Ye Zhang, Jianfeng Chen, Jiangdong Qiu, Keting Huang, Mindan Wu, Chunlin XiaExpression of IDH1 in U87MG vector control stable cells, IDH1 wild type stable cells and IDH1 R132H stable cells were test by western blotting with anti-Flag antibody.
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World Health OrganizationIDH 1 R 132H plasmidisocitrate dehydrogenase 1IDH 1 R 132H mutationActivating AKT-mTOR Signaling PathwayGSH quantification assays5 FU treatment5- FU treatmentU 87MG cellsIIIDH 1NADPcell proliferationNADPHcell migrationIDH 1 R 132H Mutation Enhances Cell MigrationIHD 1 R 132HIDH 1 R 132HMTTscratch repair assayIDH R 132H5- FU Treatmentsensitizes gliomas cells
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