Erratum: Niflumic Acid Reduces Histamine-Induced Interleukin-6 and -8 Expression in Human Conjunctival Epithelial Cells
2017-07-25T13:45:17Z (GMT) by
<b><i>Background/Aims:</i></b> Histamine remains the main mediator of allergic conjunctivitis and induces interleukin (IL)-6 and IL-8 production in human conjunctival epithelial cells (HCEC). The purpose of the present study was to determine whether histamine induced IL-6 and IL-8 expression in HCEC, and to describe the relationship between human calcium-activated chloride channel (hCLCA) 1 activity and IL-6 and IL-8 expression. <b><i>Methods:</i></b> HCEC were isolated from human conjunctiva and cultured at an air-liquid interface. <i>IL-6</i> and <i>IL-8</i> mRNA expression was examined by real-time PCR, and IL-6 and IL-8 secretion was measured by ELISA after stimulation of HCEC with histamine. <b><i>Results:</i></b> Histamine upregulated <i>IL-6</i> and <i>IL-8</i> expression and IL-6 and IL-8 secretion in a dose-dependent manner in HCEC. Niflumic acid (NFA), an hCLCA blocker, reduced histamine-induced IL-6 and IL-8 expression. <b><i>Conclusion:</i></b> Histamine-induced IL-6 and IL-8 production could be attenuated by NFA, an hCLCA blocker.