Eosinophilia does not impact on expansion of arginase-expressing AAMϕ but augments RELMα production.

Expansion of peritoneal Mϕ in BALB/c WT, ΔdblGATA deficient mice or CCR3 deficient mice (A,B) or in WT mice treated ip with rat IgG control or rat αCCR3 (C,D) at indicated time points post-ip infection with 50 BmL3. Arginase activity in PC cells from BALB/c WT, ΔdblGATA deficient or CCR3 deficient mice, WT mice treated ip with rat IgG control or rat αCCR3 (E) and expression of arg1 in purified Mϕ from WT mice treated ip with IgG control or αCCR3 (F) at indicated time points post-ip infection with 50 BmL3. F4/80 peritoneal Mϕ expression levels of RELMα in BALB/c WT or CCR3^-/- mice at +14 day post-infection with 50 BmL3 (G,H). Data from individual mice with median and interquartile range are plotted. Significant differences between groups assessed by Mann-Whitney or Kruskal-Wallis + Dunn’s tests (>2 groups). Data is from an individual experiment or pooled from 2–3 individual experiments per time-point using groups of 3–6 mice per group / time-point.