Env adaptations protect Vif-null HIV-1 from A3G deamination.

(A) Representative pseudo-single cycle infectivity data for the indicated viruses produced in SupT11 cells stably expressing A3G. Infectivity data report the average +/- SD (n = 3). Immunoblots of the indicated proteins in viral particles following p24 normalization and producer cells are shown below for one representative experiment. (B) G-to-A mutation loads in proviral DNA from viruses originally produced in SupT11 cells expressing A3G (mean +/- SD of 3 independent experiments with a minimum of 10 sequences or 5,640 bp analyzed per condition). (C) Images of ethidium bromide-stained agarose gels containing pol 3D-PCR products recovered from CEM-GFP cells infected with the indicated viruses produced in SupT11-A3G cells. Untransfected proviral plasmid (pIIIB) and DNA from uninfected CEM-GFP (mock) are controls.