Supporting Information S1 - Endothelial Progenitor Cells (EPCs) as Gene Carrier System for Rat Model of Human Glioma

To determine the distribution of IV administered EPCs to different organs in tumor bearing rats, In-111-oxine labeled EPCs were administered IV on day 21 following tumor implantation. Cells were labeled with In-111-oxine using a standard method keeping the In-111 dose at 2–3 bq/cell. SPECT images were obtained at different time points, from 1 hour to 72 hours. To differentiate between the activity of In-111 and that of In-111 labeled cells on SPECT images, animals bearing glioma that received In-111-oxine alone also underwent SPECT images were obtained at different time points. Supporting information Figures S1 and S2 show the biodistribution of In-111-oxine and In-111 labeled EPCs in different organs and in the tumors.

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