pone.0171274.g005.tif (1018.21 kB)
Electrophorograms of the RT-PCR products of NER genes in Ca. Vesicomyosocius okutanii (Vok) and the symbiont of C. pacifica (Cpac_S).
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posted on 2017-02-15, 19:49 authored by Shigeru Shimamura, Takashi Kaneko, Genki Ozawa, Mamiko Nishino Matsumoto, Takeru Koshiishi, Yoshihiro Takaki, Chiaki Kato, Ken Takai, Takao Yoshida, Katsunori Fujikura, James P. Barry, Tadashi MaruyamaA, Cpac_S; B, Vok. Expression of NER-related genes was analyzed by RT-PCR. Lane 1, uvrA; lane 2, uvrB or corresponding DNA region; lane 3, uvrC or corresponding DNA region; lane 4, uvrD; lane 5, uvrDp; lane 6, recA; lane 7, mfd; lane 8, 16S rRNA gene; lane 9, 16S rRNA gene negative control (with RNase treatment before RT PCR). M, molecular markers. Primers and the predicted lengths of amplicons are shown in S3 Table. Red arrowheads indicate the position of a band where no signal was detected.
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RT-PCRreductive genome evolutionGCNER genesRGEDNA repair genes7 clade II symbiontsNucleotide Excision Repairclade IIclade II symbiontstranscription-polymerase chain reactionvesicomyid clam symbiontsuvrD pvesicomyid clams lacknucleotide excision repairvesicomyid clams Intracellular thioautotrophic symbionts
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