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Electrochemical Magnetoimmunosensing Strategy for the Detection of Pesticides Residues
journal contribution
posted on 2006-03-15, 00:00 authored by E. Zacco, M. I. Pividori, S. Alegret, R. Galve, M.-P. MarcoA novel electrochemical immunosensing strategy for the
detection of atrazine based on magnetic beads is presented. Different coupling strategies for the modification
of the magnetic beads with the specific anti-atrazine
antibody have been developed. The immunological reaction for the detection of atrazine performed on the
magnetic bead is based on a direct competitive assay
using a peroxidase (HRP) tracer as the enzymatic label.
After the immunochemical reactions, the modified magnetic beads can be easily captured by a magnetosensor
made of graphite−epoxy composite, which is also used
as the transducer for the electrochemical immunosensing.
The electrochemical detection is thus achieved through a
suitable substrate and mediator for the enzyme HRP. The
electrochemical approach is also compared with a novel
magneto-ELISA based on optical detection. The performance of the electrochemical immunosensing strategy
based on magnetic beads was successfully evaluated using
spiked real orange juice samples. The detection limit for
atrazine using the competitive electrochemical magnetoimmunosensing strategy with anti-atrazine-specific antibody covalent coupled with tosyl-activated magnetic
beads was found to be 6 × 10-3 μg L-1 (0.027 nmol L-1).
This strategy offers great promise for rapid, simple, cost-effective, and on-site analysis of biological, food, and
environmental samples.
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Keywords
electrochemical immunosensing strategyelectrochemical detectionelectrochemical approachelectrochemical immunosensingjuice samplesnovel electrochemical immunosensing strategyenzyme HRPelectrochemical magnetoimmunosensing strategyimmunochemical reactionsatrazinedetection limitbeadsElectrochemical Magnetoimmunosensing StrategyPesticides Residues
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