Eight identical multiplexed PCR reactions performed with TTC.

<p>Lane 1: ladder. Lanes 2 and 3: amplicons produced by the commercial thermal cycler (run time of 84 min). Lane 4: ladder. Lanes 5 to 12: amplicons produced by the TTC using a 180s/35x(15s/2.5s/20s) protocol (run time of 28 min). Lane 13: ladder.</p>