Effects of sclerostin on the early stage of chondrocyte differentiation.

ATDC5 cells were cultured under chondrogenic conditions, and 20 ng/ml sclerostin were added to the culture medium. (A) Absorbance at 640 nm of sGAG in the culture solution. N = 4, *P<0.05 vs control. (B) Relative mRNA expressions of Sox9, Col2a1, and Col10a1 at 3 weeks of culture. N = 4, *P<0.05 vs control. (C) Relative mRNA expressions of Wnt3a, Wnt5a, LRP5, LRP6, Axin2, and β-catenin at 3 weeks of culture. N = 4, *P<0.05 vs control.