Effects of pan- and isoform-specific inhibitors on protein kinase activity.

A) Representative autoradiograph scans showing relative intensity of phosphorylation in the presence of pan-inhibitors LY294002 and wortmannin, and p110α-specific inhibitors A66 and PIK-75, p110β-specific inhibitor TGX221 and p110γ-specific inhibitor AS252424. Graphs summarise the results of N≥3 experiments for H1047R (black bars) and E545K (white bars) to p110α (hatched bars), p110β (cross-hatched bars) and p110γ (horizontal stripe bars) B) IC50 curves for selected inhibitors PIK-75, LY294002, A66 and wortmannin against p110α, H1047R and E545K.