Effects of fenretinide (4-HPR) on Wnt3a/β-catenin pathway.

WB analysis for proteins involved in Wnt3a/β-catenin pathway show decreased level of Wnt3a in DAOY cells (A) treated for 24 hours with 4-HPR at the highest dose while Wnt3a down-regulation started at 2.5 μM in ONS-76 cells (B). Axin-1 and pGSK3β decreased in DAOY and ONS-76 cells treated as above (C-F). Decreased levels of β-catenin was observed at 6 and 24 hours following 4-HPR treatment in DAOY and ONS-76 cells (G, H).