Effects of IL-1β, tumor necrosis factor alpha (TNF-α) and IL-17 used alone and in combination on c-mRNA expression

<p><b>Copyright information:</b></p><p>Taken from "Increased AP-1 and NF-κB activation and recruitment with the combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes"</p><p>Arthritis Research & Therapy 2004;6(3):R190-R198.</p><p>Published online 26 Feb 2004</p><p>PMCID:PMC416439.</p><p>Copyright © 2004 Granet et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> After 12 hours of culture without FCS, rheumatoid arthritis fibroblast-like synoviocytes were stimulated for 20 min with individual or combined cytokines at low concentrations: IL-1 (10 pg/ml), TNF-α (10 pg/ml) and IL-17 (50 ng/ml). Cells were lyzed and total mRNA was extracted. RT-PCR was performed as described in Materials and methods. The intensity of the respective and c-bands was measured by densitometry. Results were expressed as the ratio between c-values divided by those of the internal control and were the mean of three independent experiments. CT, control.