Effects of ACT and AVP on AQP4 expression in astrocytes.

<p>Time course of the expression of AQP4 mRNA and protein in astrocytes at 6, 12, 24, and 48 h after treatment with AVP and combination of ACT and AVP. (A, B) The expression of AQP4 mRNA was analyzed by quantitative real-time RT-PCR and compared to the respective untreated controls. The expression, standardized by the level of GAPDH, is shown as folds of expression. (C, D) The AQP4 protein level was analyzed by Western blotting. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p>