Effectiveness of magnetic fluid hyperthermia against <i>Candida albicans</i> cells

<p><i>Candida albicans</i> is one of the most frequently isolated fungal pathogens causing opportunistic infections in humans. Targeted magnetic fluid hyperthermia (MFH) is a promising method in thermal therapy facilitating selective heating of pathogen cells like <i>C. albicans</i>. In the paper, we used <i>meso</i>-2,3-dimercaptosuccinic acid (DMSA)-coated magnetic nanoparticles (MNPs) and functionalised anti-<i>C. albicans</i> immunomagnetic nanoparticles (IMNPs) to investigate the potential of MFH in combating <i>C. albicans</i> cells <i>in vitro</i>. Using Mössbauer spectroscopy it was found that synthesised MNPs exhibited superparamagnetic phenomena. On the basis of calorimetric experiments, the maximum SAR (specific absorption rate) was found and a proper concentration of MNPs was established to control the temperature. MFH based on both DMSA-coated MNPs and functionalised anti-<i>C. albicans</i> IMNPs was more effective in combating <i>C. albicans</i> cells <i>in vitro</i> than thermostat hyperthermia. Especially promising results were obtained using functionalised IMNPs, which eradicated most of the pathogen colonies at the temperature of 43 °C.</p>