Fig 6.tif (532.21 kB)

Effect of immunobiotic bifidobacteria in antiviral immune response of porcine intestinal epithelial cell line (PIE cells).

figure

posted on 2016-03-29, 08:02 authored by Takamasa Ishizuka, Paulraj Kanmani, Hisakazu Kobayashi, Ayako Miyazaki, Junichi Soma, Yoshihito Suda, Hisashi Aso, Tomonori Nochi, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Julio Villena, Haruki Kitazawa

PIE cells were pre-treated with B. infantis MCC12 or B. breve MCC1274 for 48 hours, and then challenged with rotavirus OSU. The expression level of IFN-β, MxA, RNaseL, RIG-I, TLR3, A20, and cytokines (CXCL10, IL-6, IL-8, MCP-1) were quantified by RT-PCR after 6 and 12 hours. The results represent data from three independent experiments and are expressed as mean ± S.D. Asterisks indicate significant differences: p <0.05 (*), p <0.01 (**), and p < 0.001(***).