Effect of <i>sinR</i> or <i>sinR’</i> overexpression in the R20291 strain.

<p>The <i>sinR</i> or the <i>sinR’</i> gene was cloned under tetracycline-inducible promoter and the resulting plasmid constructs were introduced into wildtype (WT) R20291 strain for overexpression. <b>(A)</b> Toxin ELISA, <b>(B)</b> Motility assay <b>(C)</b> Sporulation frequency. The data shown are means ± standard errors of three biological replicates. Statistical analysis was performed using one way-ANOVA with Dunnett’s multiple comparisons test comparing values to the average of the parent with vector control (***<0.0005, *< 0.05 <i>p</i>-value).</p>