Effect of sinR or sinR’ overexpression in the R20291 strain.

The sinR or the sinR’ gene was cloned under tetracycline-inducible promoter and the resulting plasmid constructs were introduced into wildtype (WT) R20291 strain for overexpression. (A) Toxin ELISA, (B) Motility assay (C) Sporulation frequency. The data shown are means ± standard errors of three biological replicates. Statistical analysis was performed using one way-ANOVA with Dunnett’s multiple comparisons test comparing values to the average of the parent with vector control (***<0.0005, *< 0.05 p-value).