Effect of endothelium removal and CaSR inhibition on vascular response to R-568.

<p>(A) MVB isolated from 8-week old WKY (closed symbols) and SHR (open symbols) were stimulated with increasing concentrations of R-568 in the presence (circles) or in the absence (squares) of endothelium. Results shown are the mean ± SEM of 3 (WKY) and 3 (SHR) independent experiments. Data from each curve were normalized as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0202354#pone.0202354.g001" target="_blank">Fig 1</a>. (B) MVB isolated from 8-week old WKY (closed symbols) and SHR (open symbols) were stimulated with increasing concentrations of R-568 in the absence (circles) or after pretreatment with (diamonds) Calhex-231 (3 μM/20 min). Results are the mean ± SEM of 3 WKY and 3 SHR independent experiments. Data from each curve were normalized as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0202354#pone.0202354.g001" target="_blank">Fig 1</a>. *p < 0.05 <i>vs</i> respective CTRL curve.</p>