Fig 6.tif (563.75 kB)

Effect of ROS modulation on sirtuin activity.

figure

posted on 2017-10-23, 18:30 authored by Arne Björn Potthast, Theresa Heuer, Simone Johanna Warneke, Anibh Martin Das

(A) Relative amount of reactive oxygen species (ROS) in COX-deficient cells compared to control cells (B) Detection of relative amount of ROS in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol) (C-D) Enzymatic activities of SIRT1 (C) and SIRT 3 (D) in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol) (E-F) Relative transcript levels of SIRT1 (E) and SIRT 3 (F) after treatment with a ROS scavenger (tocopherol) or ROS inducers (paraquat, diclophenac) in control fibroblasts (G-H) Relative protein amount of SIRT1 (G) and SIRT 3 (H) in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol); (n = 3; all data are shown in mean ± SD; * p < 0.05).