jf5026983_si_001.pdf (165.22 kB)
Effect of Exogenous Amylolytic Enzymes on the Accumulation of Chlorogenic Acid Isomers in Wounded Potato Tubers
journal contribution
posted on 2014-08-06, 00:00 authored by Ana Mariel Torres-Contreras, Vimal Nair, Luis Cisneros-Zevallos, Daniel A. Jacobo-VelázquezPotato tubers under wounding stress
synthesize chlorogenic acid
isomers, which are phenolic compounds that prevent chronic diseases.
The biosynthesis of phenolic compounds in plants requires aromatic
amino acids that are produced from sugars. Therefore, in this study,
we hypothesized that the wound-induced accumulation of chlorogenic
acid isomers in potatoes could be enhanced if the availability of
sugars is increased by exogenous amylolytic enzymes applied to the
surface of the site of wounding. To test this hypothesis, wounded
potatoes stored at 20 °C were treated with amylolytic enzymes
(pullulanase and amyloglucosidase, 282 units/mL, 10 mL/kg) after being
stored for 0 (E0h), 48 (E48h), or 96 h (E96h). The highest level of
accumulation of total chlorogenic acid isomers (∼210% higher
than that of time 0 h samples) was observed after storage for 120
h for the E96h treatment. The results suggest that increasing the
availability of carbon sources needed for the biosynthesis of phenolic
compounds would trigger their accumulation in wounded plants.