tx6b00277_si_001.pdf (2.49 MB)
EPR Imaging Spin Probe Trityl Radical OX063: A Method for Its Isolation from Animal Effluent, Redox Chemistry of Its Quinone Methide Oxidation Product, and in Vivo Application in a Mouse
journal contribution
posted on 2016-11-07, 00:00 authored by Maciej Serda, Yen-Ku Wu, Eugene D. Barth, Howard J. Halpern, Viresh H. RawalWe report herein a method for the
recovery, purification, and application
of OX063, a costly, commercially available nontoxic spin probe widely
used for electron paramagnetic resonance (EPR) imaging, as well as
its corresponding quinone methide (QM) form. This precious probe can
be successfully recovered after use in animal model experiments (25–47%
recovery from crude lyophilizate with 98.5% purity), even from samples
that are >2 years old. Significantly, the recovered trityl can
be
reused in further animal model EPR imaging experiments. The work also
describes support for the observed formation of an air-sensitive radical
derived from the QM under reducing conditions.