EML1-NTD expressing cell in Anaphase

2018-12-03T09:41:43Z (GMT) by Marie Nugent Laura O'Regan
<b>Lay summary and brief biological or clinical significance</b><div>A human cancer cell is shown in the act of dividing. This cancer cell has been experimentally manipulated to contain a protein that drives the spread of the cancer through the body. Understanding the process of cancer cell division and the molecular pathways involved allows us to identify new strategies for the selective killing of rapidly dividing cancer cells.<br></div><div><br></div><div><b>Technical summary</b><br></div><div>Human U2OS osteosarcoma cells were transfected with YFP-EML1-NTD and fixed in ice-cold methanol and stained with antibodies against GFP (green) and α-tubulin (red). DNA was stained with Hoechst 33258 (blue).<br></div><div><br></div><div>Imaging Details<br></div><div><table> <tr> <td> <p>Hardware</p> </td> <td> <p>Leica Confocal TCS SP5</p> </td> </tr> <tr> <td> <p>Image Capture Software</p> </td> <td> <p>Leica Software</p> </td> </tr> <tr> <td> <p>Laser(s) used<br> (if applicable)</p> </td> <td> <p>50 mW diode 405 nm; 100 mW Argon laser 488; 10 mW DPSS 561 nm</p> <p> </p> </td> </tr> <tr> <td> <p>Fluorophores used <br> (if applicable)</p> </td> <td> <p>Primary: GFP (rabbit); α-tubulin (mouse)</p> <p>Secondary: Alexa fluor goat anti-rabbit 488; Alexa fluor goat anti-mouse 594, Hoechst 33258</p> </td> </tr> <tr> <td> <p>Objective</p> </td> <td> <p>X63 Plan Apo</p> </td> </tr> </table><br></div>