E. Coli Growth over 4 hours

2012-04-20T20:03:42Z (GMT) by Anthony Salvagno alexandria haddad
<p>In preparation for some deuterium effects on E. coli and S. cerevisiae, I grew a starter culture and diluted it in 3 different concentrations. 1:10, 1:5, and 1:2. These dilutions were then grown at 37C for 4 hours and an absorption measurement was taken every hour. This fileset contains the raw data and some played with data, along with some figures made in Excel from the data.</p> <p>The file labeled "arb-ecoli-growth.png" is a figure made from manipulated data. I tried to combine the three data sets into one graph to see if I could extract some sort of growth information. I'm pretty sure I didn't do it right, but I included the image here nonetheless.</p> <p>In the 1:10 dilution sample, the cells would double in slightly less than one hour, every hour. In the 1:2 dilution, the growth rate was much slower, and the growth rate seemed to peak rather early in the trial. The 1:5 dilution is an overlap of growths between both the 1:10 and 1:2 dilutions. I don't know what to make of that.</p> <p>Also included in the fileset is an image of the absorbance spectrum from the nanodrop for every sample (including blanks taken every hour).</p>



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