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Dynamic BH3 profiling assay: delta priming to PUMA-BH3.
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posted on 2018-01-03, 18:39 authored by Martin Grundy, Claire Seedhouse, Thomas Jones, Liban Elmi, Michael Hall, Adam Graham, Nigel Russell, Monica PallisThe increase in cells primed to undergo mitochondrial outer membrane depolarisation (Δ priming) is measured by Cytochrome C release after 4 hour drug treatment. PUMA-BH3 was used at 3 μM. Values are corrected for Cytochrome C release with peptide only as described in the methods. (Mean+/- SD for n = 3).
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drugs sensitisingpro-apoptotic anti-leukaemic drug combinationsBH 3-only apoptosis agonists BADMCL -1-targeting MS 1-BH peptideetoposideBAD-BH 3-mediated mitochondrialNOXA target BCL -2anti-leukaemic drugs sensitisingAC 220BCL -2-targeting BAD-BH 3 peptide4 hoursmembrane permeabilisationagents sensitised cellsBH 3BCL -2DNAinhibitors pladienolide BFLT 3 inhibitor AC 220cytochrome C releasepladienolide Bcompounds ABT -199BCL -2 inhibitors ABT -199BET domain inhibitor JQ 1downregulate BCL -2BCL -2 antagonism
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