Drosophila images from predation experiment with the jumping spider salticus scenicus.

2018-02-27T20:12:09Z (GMT) by Cody Porter Ian Dworkin
Experimental Design for Analysis of Phenotypic Selection on Drosophila melanogaster Survival During Episodic Selection from Salticus Scenicus Predation.

Data collected by Cody Porter in the lab of Ian Dworkin at Michigan State University in 2011.

Images are organized in folders by whether the flies survived the experiment, or were predated upon by the spiders (dead). I have zipped up the images. Each morphological structure (wings, legs, thorax) are in their own images. For survivors each leg (1st - 3rd) has its own folder.

***How experiment was performed***

Fenn Valley (Michigan) wild type population of Drosophila melanogaster will be used in assays (flies aged between 3 and 6 days only).

24 flies per container (12 males and 12 females) randomly assorted with respect to age.

Flies spent at least 24 hours recovering from CO2 exposure while acclimating in the 24C incubator.
Flies that are collected (in vials) must be assorted with respect to sex.
*Virgin and non-virgin flies used.

A.M. Set-up

Plastic “cages” washed with 70 % ethanol followed by a rinsing with RO H20.
1 Spider per cage (begin with adults only and use juveniles as excess flies allow).
Acclimate spider in plastic cage with synthetic plant and moist kim wipe for 24 hours in 24c incubator.
Introduce flies (that have been prepared and assorted as described above) into “cages” containing spiders using plastic funnel. *Be sure to make note of any dead flies in vial or escapees during this process.
Set containers in trays and into 24c incubator and allow assay to run for 24 hours.
Add one “cap” of fly food with a single yeast granule per container.
*Record everything carefully in notebook.
*Label each individual cage with a number that corresponds to the spider present in that cage (from the spider’s vial number).

A.M. (72 hours later) Take-down

Place all containers simultaneously in the 4c refrigerator for 30 minutes.
After 45 minutes, take 2 cages out of the refrigerator at a time.
For each container, count and record the number of survivors and their sex.
Place all survivor carcasses (from all containers) into a single Eppendorf vial with ethanol and all dead carcasses (from all containers) into a single, separate Eppendorf vial with ethanol.
*If juveniles are used, place carcasses in separate, labeled vials with ethanol, just as with adults.

Miscellaneous Notes:

Have everyone remove mite nets prior to placing cages into incubator.
Deprive spiders of food (D. melanogaster) for 96 hours prior to assay.

***How traits were imaged (zoom, scope, etc...)***

Microscope - Leica M125 with a Leica DFC400 Camera

- Zoom Drive (1.00X)

Magnifications used
50.0x -- All three legs on left side (5.0)
40.0x -- Both wings (4.0)
25.0x -- Thorax (2.5)

-Image Formats

Captured Format -- 1392x1040, Full Frame HQ

Live Format -- 1392x1040, Full Frame

Image Type -- Greyscale

Iris Manual -- 27%

***Naming convention for images***

Salticus scenicus_Survivorship_Sex_Experiment#_Trait_Side(or leg #)

Example - Ss_S_F_1_W_L

=Salticus scenicus;survivor;female;Experiment#1;Wing;Left

**A 'X' next to, or in place of, any piece of information denotes missing information.

Example - Ss_D_X_2_LX_2

=Salticus scenicus;dead;sex unknown; Experiment#2;Leg (side of body unknown);leg#2