Displacements of fluorescent beads induced by a GFP-HUVEC on a 1.3 kPa PAA gel.
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posted on 2015-12-07, 09:13 authored by Alvaro Jorge-Peñas, Alicia Izquierdo-Alvarez, Rocio Aguilar-Cuenca, Miguel Vicente-Manzanares, José Manuel Garcia-Aznar, Hans Van Oosterwyck, Elena M. de-Juan-Pardo, Carlos Ortiz-de-Solorzano, Arrate Muñoz-Barrutia(a) Maximum intensity projection of the fluorescent image of the HUVEC. (b) Pseudo-color image showing the fluorescent beads at the gel surface. The beads in the unstressed and stressed gel are pseudo-colored in red and green, respectively. The contrast of the pseudo-color image has been modified to highlight the areas with bead displacements. The scale bar represents 30μm.
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3 D fieldsFree Form Deformationpolyacrylamide substratedeformable meshdeformation fieldPIVblock-matching schemenon-rigid image registration processFFD algorithmnon-uniform deformationsFFD approachParticle Image VelocimetryTFMtraction recoveryTraction Force Microscopy Traction Force Microscopyresults show
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