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Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli
journal contribution
posted on 2015-03-06, 00:00 authored by Steffen Krauser, Thomas Hoffmann, Elmar HeinzleWe report on tailored regeneration
modules for the essential cofactors
ATP and NADPH as well as for SAM supply, adapted to support biocatalytic
multistep synthesis of oxytetracycline (OTC) in permeabilized cells
of E. coli. ATP is regenerated from
ADP and AMP by the use of polyphosphate. The efficiency of this system
was proven with permeabilized cells producing flaviolin. NADPH is
regenerated from NADP+ by oxidation of phosphonate. A constant
level of 0.7 mg/L of SAM is provided by the ligation of ATP and methionine.
OTC is synthesized at a rate of 2.05 ng/(h (g of the combined biocatalyst)),
reaching a final concentration of 0.9 mg/L.