Direct reprograming of MEFs into chemically induced neurons (CiNeurons).
(A) Schematics of experimental setup of direct reprograming of MEFs to CiNeurons by small molecule cocktail. (B) Cell morphology of WT, HET and KO MEFs and the corresponding CiNeurons. Scale bar: 50 μm. (C) qPCR quantification of relative gene expression in MEFs and CiNeurons. Nono housekeeping gene was used for normalization, and the expression level of WTN was set as 1 for each gene. Data are the summary of 2 independent batches of reprogramed cells. Error bar: S.E.M. (D) Immunofluorescence of Synapsin I and MAP2 staining in CiNeurons, Scale bar: 50 μm. (E) Confocal images of Tuj1 and NeuN staining in CiNeurons, Scale bar: 50 μm.