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Differential effects of energy stress on AMPK phosphorylation and apoptosis in experimental brain tumor and normal brain-1
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posted on 2011-12-31, 11:19 authored by Purna Mukherjee, Tiernan J Mulrooney, Jeremy Marsh, Derek Blair, Thomas C Chiles, Thomas N Seyfried4 hours. a) Cell lysates were probed with specific phosphorylated and non-phosphorylated antibodies for western blot as indicated. Dose dependent increase in AMPK phosphorylation was evident in CT-2A and astrocytes but caspase-3 and PARP cleavage was noted only in AICAR treated CT-2A cells. b) Cells were treated with 1.0 mM AICAR in glucose, glutamine and serum containing media for 24 hours and treated with Annexin V and PI stain as described in methods. Labeled cells were analyzed by flow cytometry. 8–10% apoptotic cells were noted in CT-2A cells treated with AICAR compared to untreated cells. No significant cell-death was noticed in treated astrocytes. All experiments were done in triplicate.
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Taken from "Differential effects of energy stress on AMPK phosphorylation and apoptosis in experimental brain tumor and normal brain"
http://www.molecular-cancer.com/content/7/1/37
Molecular Cancer 2008;7():37-37.
Published online 12 May 2008
PMCID:PMC2397440.
