Diagram of sequenced adaptor-antibody-adaptor constructs.

A) scFv library (gray), comprising heavy (VH) and light chain (VL) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). B) VH nanobody library (gray), comprising heavy chain (VH) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). The forward read (R1) uses SBS3 sequencing primer (Illumina), while the reverse read (R2) uses SBS12 primer (Illumina). iS1 and iS2 = index/shifter sequences.