am5b08314_si_001.pdf (874.86 kB)
Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G‑Quadruplex Formation
journal contribution
posted on 2015-11-04, 00:00 authored by Ka-Ho Leung, Bingyong He, Chao Yang, Chung-Hang Leung, Hui-Min David Wang, Dik-Lung MaWe report a label-free, structure-independent
luminescent-sensing
platform for metal ions, proteins, and small molecules utilizing an
Ir(III) complex, terminal deoxynucleotidyl transferase (TdT), and
a structure-folding aptamer. A novel G-quadruplex-selective Ir(III)
complex was identified to detect the nascent G-quadruplex motifs with
an enhanced luminescence response. Unlike most label-free DNA-based
assays reported in the literature, this sensing platform does not
require a specific secondary structure of aptamer, thus greatly simplifying
DNA design. The detection platform was demonstrated by the detection
of K+ ions, thrombin, and cocaine as representative examples
of metal ions, proteins, and small molecules.