figshare
Browse
mz7b00242_si_001.pdf (297.09 kB)

Development of Excipient-Free Freeze-Dryable Unimolecular Hyperstar Polymers for Efficient siRNA Silencing

Download (297.09 kB)
journal contribution
posted on 2017-06-19, 13:05 authored by Liang Zhao, Xiang Wu, Xiaofeng Wang, Chunying Duan, Hongyan Wang, Amol Punjabi, Yang Zhao, Yuanwei Zhang, Zuoshang Xu, Haifeng Gao, Gang Han
We designed a unimolecular hyperstar polymer for efficient small interfering RNA (siRNA) delivery that can be processed under repeated lyophilization and reconstitution without the need of any excipient. The hyperstar polymer contains a biodegradable hyperbranched core and is bound to siRNA through its thousands of cationic arms that radiate from its core. The siRNA/hyperstar complexes showed siRNA transfection efficiency that was superior to that of Lipofectamine2000 in regard to the gene for human Cu, Zn superoxide dismutase 1 (SOD1), whose mutation causes familial amyotrophic lateral sclerosis. More importantly, hyperstar polymers as unimolecular containers minimized the multipolymer cross-interaction during lyophilization, and this maintained the uniquely high transfection efficiency of the siRNA/hyperstar complexes after repeated freeze-drying and reconstitution without the conventional need for excipient.

History