figshare
Browse
1/1
9 files

Determination of Colchicine in Pharmaceutical Formulations and Urine by Multiple-Pulse Amperometric Detection in an FIA System Using Boron-Doped Diamond Electrode

dataset
posted on 2018-08-22, 02:45 authored by Débora A. R. Moreira, Fernando M. de Oliveira, Dilton M. Pimentel, Tiago J. Guedes, Rita C. S. Luz, Flávio S. Damos, Arnaldo C. Pereira, Rodrigo A. B. da Silva, Wallans T. P. dos Santos

This work presents a simple and fast method for colchicine (CO) determination in pharmaceutical formulations and urine by multiple pulse amperometry (MPA) with flow injection analysis (FIA) system using a boron-doped diamond electrode. In optimized conditions, it was possible to quantify CO in urine samples without interference of uric acid and ascorbic acid. The working linear range for CO quantification was achieved from 1.0 × 10-7 to 0.5 × 10-3mol L-1 with a low limit of detection of 2.14 × 10-8mol L-1. Furthermore, the proposed method showed high repeatability for 10 consecutive injections of 1.0 × 10-4mol L-1 CO (relative standard deviation = 1.28%) and good analytical frequency (30 determinations per hour). The addition and recovery studies in all pharmaceutical samples were approximately 100% and the results for CO determination were compared by high performance liquid chromatography (HPLC) with UV detection.

History

Usage metrics

    Journal of the Brazilian Chemical Society

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC