Detailed virology and serology of 4 RM infected after vaginal ZIKV inoculation in relation to the menstrual cycles of each animal.

vRNA levels in plasma, CVL, urine and packed blood cells (whole blood) on the left y-axis. Plasma anti-ZIKV antibody levels are indicated on the right y-axis. The shaded vertical boxes indicate the days menstrual blood was detected by visual inspection, with the first day of bleeding designated Day 1 of the menstrual cycle. A) RM 37072 infected after 1 low dose (10⁴ pfu) inoculation in the peri-ovulatory phase of her menstrual cycle. B) RM 37182 infected after 1 moderate dose (10⁵ pfu) inoculation in the follicular phase of her menstrual cycle. C) RM 38728 infected after the second of 2 high dose (10⁶ pfu) inoculations in the peri-ovulatory phase of her menstrual cycle. D) RM 40125 infected after 5^th of 5 high dose (10⁶ pfu) inoculations in the luteal phase of her menstrual cycle.