Deconvolved Bead Image

2014-04-18T00:30:59Z (GMT) by Brian Northan
<p>Deconvolved bead image.  Original dataset was acquired by Kurt Thorn UCSF and is in  100xPSF_deconvolved_nc_200.ome.tif.zip.</p> <p>Deconvolved using 200 iterations of non-circulant Richardson Lucy.  The PSF was generated using COSMOS software using the Gibson Lanni method using  1.4 NA, the pixel size is 65 nm and the Z spacing is 200 nm, sample RI of 1.4, and depth of 1 micron.</p> <p>Code was implemented as Image2 plugins and can be found here: https://github.com/bnorthan/projects.</p>

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CC BY 4.0