100xPSF_deconvolved_nc_200.ome.tif.zip (10.61 MB)

Deconvolved Bead Image

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posted on 18.04.2014, 00:30 by Brian Northan

Deconvolved bead image.  Original dataset was acquired by Kurt Thorn UCSF and is in  100xPSF_deconvolved_nc_200.ome.tif.zip.

Deconvolved using 200 iterations of non-circulant Richardson Lucy.  The PSF was generated using COSMOS software using the Gibson Lanni method using  1.4 NA, the pixel size is 65 nm and the Z spacing is 200 nm, sample RI of 1.4, and depth of 1 micron.

Code was implemented as Image2 plugins and can be found here: https://github.com/bnorthan/projects.

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