Deconvolved Bead Image
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Deconvolved bead image. Original dataset was acquired by Kurt Thorn UCSF and is in 100xPSF_deconvolved_nc_200.ome.tif.zip.
Deconvolved using 200 iterations of non-circulant Richardson Lucy. The PSF was generated using COSMOS software using the Gibson Lanni method using 1.4 NA, the pixel size is 65 nm and the Z spacing is 200 nm, sample RI of 1.4, and depth of 1 micron.
Code was implemented as Image2 plugins and can be found here: https://github.com/bnorthan/projects.