ValidationReports-6GC9-6GCA-6GCB-6GCC.pdf (1.49 MB)
Dataset for: Trametes versicolor Glutathione Transferase Xi 3, a dual Cys-GST with catalytic specificities of both Xi and Omega classes
dataset
posted on 2018-09-06, 12:47 authored by Mathieu Schwartz, Thomas Perrot, Aurélie Deroy, Thomas Roret, Melanie Morel-Rouhier, Guillermo Mulliert, Eric Gelhaye, Frédérique Favier, Claude DidierjeanGlutathione transferases (GSTs) from the Xi and Omega classes have a catalytic cysteine residue which gives them reductase activities. Until now, they have been assigned distinct substrates. While Xi GSTs specifically reduce glutathionyl-(hydro)quinones, Omega GSTs are specialized in the reduction of glutathionyl-acetophenones. Here we present the study of TvGSTX1 and TvGSTX3 isoforms from the wood-degrading fungus Trametes versicolor. TvGSTX1 reduces GS-menadione as expected while TvGSTX3 reduces both Xi and Omega substrates. A structural analysis indicates a broader active site for TvGSTX3 due to specific differences in the nature of the residues situated in the C-terminal helix α9. This feature could explain the catalytic duality of TvGSTX3. Based on phylogenetic analysis we propose that this duality might exist in other fungi.
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Categories
- Animal physiology - biophysics
- Human biophysics
- Synthetic biology
- Biochemistry and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Virology
- Receptors and membrane biology
- Bioinformatics and computational biology not elsewhere classified
- Immunology not elsewhere classified
- Neurosciences not elsewhere classified
- Cell development, proliferation and death
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Structural biology (incl. macromolecular modelling)
Keywords
Glutathione transferaseDual ActivityCrystallographyBiophysicsSynthetic BiologyBiochemistryPlant BiologyVirologyReceptors and Membrane BiologyComputational BiologyImmunologyNeuroscienceCell Development, Proliferation and DeathMolecular BiologyEvolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyStructural Biology
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