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Dataset for: A novel chitin binding mode of the chitin binding domain of chitinase A1 from Bacillus circulans WL-12 revealed by solid-state NMR
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posted on 2018-09-01, 09:09 authored by Hiroki Tanaka, Hideo Akutsu, Izumi Yabuta, Masashi Hara, Hayuki Sugimoto, Takahisa Ikegami, Takeshi Watanabe, Toshimichi FujiwaraChitin-binding domain of chitinase A1 (ChBDChiA1) is characteristic because it binds only to insoluble crystalline chitin. While binding sites of major carbohydrate-binding modules carry multiple aromatic rings aligned on a surface, lethal mutations for ChBDChiA1 were reported only at W687, a location completely different from the site mentioned above, in spite of their similar main chain folds. Here, the structural mechanism underlying its crystalline chitin-binding was uncovered by solid-state NMR. Based on 13C- and 15N-signal assignment of microcrystalline ChBDChiA1, the chemical shift perturbation on chitin-binding was carefully examined. The perturbation was greatest at W687 and non-aromatic residues surrounding it, revealing their direct involvement in chitin-binding. These residues and Q679 should provide a novel chitin-binding platform parallel to the W687 ring.
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Categories
- Animal physiology - biophysics
- Human biophysics
- Synthetic biology
- Biochemistry and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Virology
- Receptors and membrane biology
- Bioinformatics and computational biology not elsewhere classified
- Immunology not elsewhere classified
- Neurosciences not elsewhere classified
- Cell development, proliferation and death
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Structural biology (incl. macromolecular modelling)
Keywords
insoluble chitincarbohydrate-binding proteinNMRBiophysicsSynthetic BiologyBiochemistryPlant BiologyVirologyReceptors and Membrane BiologyComputational BiologyImmunologyNeuroscienceCell Development, Proliferation and DeathMolecular BiologyEvolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyStructural Biology
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